元件指南
VectorBuilder提供了許多常用的載體元件,可供用戶在設(shè)計載體時進行選擇。下表按類型提供了這些常用元件的詳細信息。
最小啟動子
姓名 | 描述 | Application Notes | References | 序列 |
---|---|---|---|---|
Hsp68 | Mouse heat-shock protein 68 minimal promoter | Strong core promoter; suitable for driving gene expression in vivo. | Development. 105:707 (1989) | View |
miniCMV | Human cytomegalovirus minimal promoter | Weak basal activity | Gene Ther. 16:43 (2009) | View |
minP | Synthetic minimal promoter composed of TATA box and transcription start site | Basal activity weaker than miniCMV | Developed by Promega | View |
SCP1 | Super core promoter 1 minimal promoter, synthetic core promoter | Basal activity stronger than miniCMV but weaker than Hsp68 | Nat Methods. 3:917 (2006) | View |