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元件指南

VectorBuilder提供了許多常用的載體元件，可供用戶在設(shè)計載體時進行選擇。下表按類型提供了這些常用元件的詳細信息。
Popular shRNAs
姓名靶序列Target GeneTarget PositionReferences序列
Scramble[shRNA#1]CCTAAGGTTAAGTCGCCCTCGNone in human and mouseCancer Res. 66:9270 (2006) View
Scramble[shRNA#2]CAACAAGATGAAGAGCACCAATurboGFP; none in human and mouseOncogene. 30:1551 (2011); Science. 343:309 (2014) View
EGFP[shRNA#1]CGACGTAAACGGCCACAAGTTEGFP63-83The RNAi Consortium ID: TRCN0000072193 View
EGFP[shRNA#2]ACGTCTATATCATGGCCGACAEGFP449-469The RNAi Consortium ID: TRCN0000231756 View
LacZ[shRNA#1]GTCGGCTTACGGCGGTGATTTLacZ1734-1754The RNAi Consortium ID: TRCN0000072242 View
LacZ[shRNA#2]GTTCCGTCATAGCGATAACGALacZ1908-1928The RNAi Consortium ID: TRCN0000072238 View
Luciferase[shRNA#1]ATGTTTACTACACTCGGATATFirefly luciferase745-765The RNAi Consortium ID: TRCN0000231737 View
Luciferase[shRNA#2]TGTCCGGTTATGTAAACAATCFirefly luciferase1193-1213The RNAi Consortium ID: TRCN0000072258 View
Scramble[miR30-shRNA#1]ACCTAAGGTTAAGTCGCCCTCGNone in human and mouseDesigned by VectorBuilder View
Scramble[miR30-shRNA#2]CCAACAAGATGAAGAGCACCAATurboGFP; none in human and mouseOncogene. 30:1551 (2011); Science. 343:309 (2014) View
EGFP[miR30-shRNA#1]GCGACGTAAACGGCCACAAGTTEGFP62-83Designed by VectorBuilder View
EGFP[miR30-shRNA#2]AACGTCTATATCATGGCCGACAEGFP448-469Designed by VectorBuilder View
EGFP[miR30-shRNA#3]AAGAACGGCATCAAGGTGAACTEGFP475-496Designed by VectorBuilder View
EGFP[miR30-shRNA#4]CACATGAAGCAGCACGACTTCTEGFP232-253Designed by VectorBuilder View
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