VectorBuilder提供了許多常用的載體元件，可供用戶在設(shè)計(jì)載體時(shí)進(jìn)行選擇。下表按類型提供了這些常用元件的詳細(xì)信息。
| 姓名 | 描述 | Application Notes | References | 序列 |
|---|
| WPRE | Woodchuck hepatitis virus posttranscriptional regulatory element | Enhances virus stability in packaging cells, leading to higher titer of packaged virus; enhances higher expression of transgenes. | J Virol. 73:2886 (1999); Hum Gene Ther. 10:2295 (1999) |
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| WPRE3 | Truncated short version of woodchuck hepatitis virus posttranscriptional regulatory element (248 bp) | Contained only minimal gamma and alpha subunits of WPRE. Slightly weaker effective compared to WPRE. | Mol Brain. 7:17(2014); Nat Biomed Eng. 6:1272 (2022) |
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| oPRE | Optimized and truncated version of woodchuck hepatitis virus posttranscriptional regulatory element | The potentially oncogenic X protein ORF and X protein promoter have been removed; ATG start sites in ORFs encoding sequences greater than 25 amino acids in length have been mutated. | Gene Ther. 13:641 (2006); Hum Gene Ther Methods. 23: 73 (2012) |
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| 姓名 | 描述 | Application Notes | References | 序列 |
|---|
| unc-54 3' UTR+polyA | Myosin-4 3' UTR with downstream sequence from C. elegans | Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase; functions in somatic cells and germ cells. | Curr Biol. 18:1476 (2008) |
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| tbb-2 3' UTR+polyA | Tubulin beta-2 chain 3' UTR with downstream sequence from C. elegans | Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase; functions in somatic cells and germ cells. | Curr Biol. 18:1476 (2008) |
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