Many gene delivery experiments utilize viral vectors for efficient delivery of genetic information to target cells. To make your recombinant virus, plasmids containing your GOI and the instructions for producing the virus are propagated in bacterial...

Gel electrophoresis is a conventional method to assess the size, composition, and amount of your DNA. It is a critical step during molecular cloning and quality control. However, what is a very simple process in theory can have many assumptions and t...

When I was a graduate student, we called the lab’s PCR machine the “Magic Box,” because the reagents that we put inside could produce exactly what we were looking for, something completely unexpected, or nothing at all. If a reaction did not work, i...